How to Care for Tissue Culture Plants?

Like all other plants that are naturally grown, tissue culture plants require proper care in order to thrive and grow. This care continues until the acclimatization stage (adaption of the plants to the external environment).Tissue culture plants are grown under controlled conditions in labs. This means that they lack distinctive characteristics. They lack waxy cuticles that regulate the rate of transpiration (rate at which leaves lose moisture) and they are not able to have them on their leaves. They are therefore unable to cope with the unpredictable changes in the environment such as humidity below 70% and are more susceptible to developing diseases.It is important to properly care for cannabis tissue culture plants once they are transferred to a normal environment. How do you do this? What are the factors to consider when acclimating plants? This article will cover everything.

Why Is It So Important To Properly Acclimate Tissue Culture Plants?

Before we get to the reason acclimatization or acclimation is important, let's first understand what acclimation actually means.The final stage in tissue culture is called acclimatization. Plants with mature shoots and roots are then transferred to a potting mixture (generally made of peat or perlite) in an enclosed chamber that allows for better humidity control.Tissue culture plants are raised in controlled conditions. They must be gradually prepared to adapt to new conditions before being released to their natural environment. Tissue culture plants will die if they are exposed to extreme conditions such as heat, humidity, board light or increased temperature. They also need to be prepared for the natural environment.You wouldn't want your plants to go after waiting so long and making such an investment in time and money. Proper, gentle, and gradual adaptation of plants is crucial.

How To Deflask Tissue Culture Plants

To get maximum yield, follow the steps for removing plants from tissue culture flasks.

• In a cool, dry, and shaded place, open the tissue culture container containing the plant.
• Take the plantlet out of the container and wash away the gel that has stuck to the roots. Add some lukewarm water to loosen the gel. Next, swirl the container until the gel begins to move in a swirling motion. Next, take the plants out and wash them off gently. This process does not cause any damage to the roots of your plants.
• Next, move the plant to a mix with low nutrient requirements. The 50:50 ratio of peat to perlite is a good choice for plants. Make sure the mixture is not too wet.
• Keep the temperature of the air between 18 and 23 degrees Celsius for the first few weeks.
• When planting the plant, make sure they are securely attached to the base.
• The tray with plants should be transferred to a humidity chamber where it can maintain 70% humidity. If you don't have a humidity chamber, place a styrofoam container with two inches of glass on top. Then add sand to the bottom.
• Spray them with a moisture trap spray solution to speed up the hardening of your plants. This will decrease rapid moisture loss.
• The trays should be placed in indirect light at 20-22 degrees Celsius. Shade screens can be used to reduce high levels of light.
• These steps are essential for hardening the plants and preparing them to be outside.

Challenges That May Occur During AcclimatizationThese are some of the challenges you may face during acclimatization

• When soil is transferred to plants, high humidity or random fluctuations in humidity can cause abnormal stomatal function. Plants will become dehydrated due to rapid water loss.
• The roots of plants grown in controlled laboratory conditions are weaker. This could make it difficult for plants to obtain nutrients from the soil.
• Plantlets experience a low rate of photosynthesis due to low light intensity, low carbon dioxide concentration and high sugar levels in the media. This further slows down their growth.
• If plants are exposed to too many plant hormones, they will exhibit abnormal morphology.
• When plants are exposed to temperature fluctuations and the board light spectrum, you might notice wilting and charring.
• The death rate of tissue culture plants increases when they are exposed to soil microflora.

To grow plants on a commercial scale, tissue culture is a very popular method among growers and businesses. It is because it has many advantages over traditional methods of growing plants such as the ability to produce hybrid plants and disease-free plants.